
Research topics: Genetic analysis of reproductive behavior and cardiac rhythms
Research program: My research area is the genetics of behavior, using Drosophila as the experimental organism. I am currently using genetics and pharmacology to investigate two separate phenomena, cardiac rhythmns and female receptivity.
Heart rhythms---Cardiac rhythms of Drosophila originate in a myogenic pacemaker. My collaborator, Dr. Harold Dowse (this Department) and I have identified two ion channels central to this pacemaker: A voltage-gated "OPQ-Type" Calcium channel, and a fast Calcium-gated potassium channel encoded by the slowpoke gene. Other Potassium channels may be involved, but sodium channels do not play a role in heartbeat. We have found several mutants with severe cardiac arrhythmias: slowpoke, no-action-potential, amnesiac, Dopa decarboxylase, and ether-a-go-go. We have also found that serotonin, octopamine, norepinephrine, dopamine, and acetylcholine are cardioacceleratory, while a pentapeptide from Limulus slows heartbeat.
Female sexual receptivity---Receptivity is switched on in young adults. I am studying the genetic and endocrine program underlying the switch-on, using a combination of genetic, physiological, and ethological approaches. A few genes are known to be crucial to receptivity; I am utilizing two, apterous (ap) and icebox. apterous mutants are deficient in JH, and therefore develop receptivity very slowly. icebox, discovered to be a behavioral mutation in this lab, exerts a stronger effect on receptivity than any other known gene. I also use JH analogs and JH inhibitors in these studies. I characterize the "microresponses" of females---the frequency and details of their signals to sexually active males---by analyzing videotaped behavior.
Selected Publications
Johnson E, J Ringo, and H Dowse 1997. Modulation of Drosophila heartbeat by neurotransmitters. J. Comp. Physiol. B 167:89-97.
Johnson E, J Ringo, N Bray, and H Dowse 1997. Genetic and pharmacological identification of ion channels central to Drosophila's cardiac pacemaker. J. Neurogenet.
Kerr, C, J Ringo, H Dowse, and E Johnson 1997. icebox, a recessive X-linked mutation in Drosophila causing low sexual receptivity. J. Neurogenet.
Ringo J 1996. Sexual receptivity in insects. Ann. Rev. Entomol. 41:473-494.
Other publications from this laboratory